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Faculty
 Mark R. Terasaki
Associate Professor of Cell Biology
terasaki@neuron.uchc.edu
Areas of Interest:
I have been using advanced light microscopy techniques to investigate
intracellular organization and function. Most of my work has involved
imaging of living cells using confocal microscopy. I have recently
completed work using green fluorescent protein chimeras to label the ER
and Golgi apparatus during the cell cycle in sea urchin embryos. Three
current projects are: how cells heal wounds in the plasma membrane;
changes in ER structure during meiotic maturation; and nuclear envelope
breakdown, where we are planning to use some computer modeling and two
photon microscopy to understand steps involved in this process.
Lab Rotation Projects: Terasaki/Fein Lab
Cell Wounding Project
We have developed an electrophysiological method for studying plasma
membrane wound repair in single cells. Large plasma membrane disruptions
in starfish oocytes, made by femtosecond pulses from a Ti-Sapphire
laser, are repaired within a few seconds as shown by stabilization of
membrane electrical properties and restoration of dye exclusion. The
membrane potential after wounding is sensitive to the extracellular Cl-
concentration but not to that of Na+, K+ or H+ indicating that Cl-
permeable intracellular membranes have fused with the plasma membrane.
We believe that cell wounding provides a novel means for the
electrophysiological analysis of chloride permeable intracellular
membranes that have been translocated to the plasma membrane.
Dr. Terasaki's Departmental Web Page
Selected Publications:
Terasaki, M. 2006. Fluorescence quantitation in thick specimens, with
an application to cyclin B-GFP expression in starfish oocytes. Biol.
Cell (in press).
Fein, A., and Terasaki, M. 2005. Rapid increase in plasma membrane
chloride permeability during wound resealing in starfish oocytes. J.
Gen. Physiol. 126: 151-159.
Millard, A.C., Terasaki, M., and Loew, L.M. 2005. Second harmonic
imaging of exocytosis at fertilisation. Biophys. J. Biophys J 88:
L46-48.
Lénárt, P., Daigle, N., Bacher, C., Hand, A.R., Eils, R., Terasaki,
M., and Ellenberg, J. 2005. A contractile nuclear actin network drives
chromosome congression in oocytes. Nature 436: 812-818.
Terasaki, M., Okumura, E., Hinkle, B., and Kishimoto, T. 2003.
Localization and dynamics of Cdc2-cyclin B during meiotic reinitation in
starfish oocytes. Mol. Biol. Cell 14:4685-4694.
Slepchenko, B.M., and Terasaki, M. 2003. Cyclin aggregation and
robustness of bio-switching. Mol. Biol. Cell 14: 4695-4706.
Galbraith, J.A., and Terasaki, M. 2003. Controlled damage in thick
specimens by multiphoton excitation. Mol. Biol. Cell 14: 1808-1817.
Hinkle, B., Slepchenko, B., Rolls, M.M., Walther, T.C., Stein, P.A.,
Mehlmann, L.M., Ellenberg, J., and Terasaki, M. 2002. Chromosomal
association of Ran during meiotic and mitotic divisions. J. Cell Sci.
115: 4685-4693.
Terasaki, M., Runft, L., and Hand, A. 2001. Organization of the
endoplasmic reticulum during Xenopus oocyte maturation and egg
activation. Mol. Biol. Cell 12: 1103-1116.
Terasaki, M., Campagnola, P., Rolls, M.M., Stein, P., Ellenberg, J.,
Hinkle, B., and Slepchenko, B. 2001. A new model for nuclear envelope
breakdown. Mol. Biol. Cell 12: 503-510. |
