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Faculty
Flavia A. O'Rourke
Assistant Professor of Cell Biology
orourke@nso1.uchc.edu
- B.S., Central Connecticut State College
- M.S., University of Connecticut
- Ph.D., University of Connecticut
- Cell Biology Graduate
Program
- Not accepting students for Lab Rotations at this time
Research is focused on signal transduction in human platelets with
specific interest in the inositol phosphate signaling pathway and its
regulation. The IP3 and IP4 receptor systems are currently being
investigated to include characterization and purification using
techniques of HPLC and affinity chromatography. A HEL cell cDNA
expression library using monoclonal antibodies and oligonucleotide
probes is also being used to clone and sequence the gene for the
receptors.
Selected Publications:
O'Rourke FA, LaPlante JM, Feinstein MB. (2003) Antisense-mediated
loss of calcium homoeostasis endoplasmic reticulum protein (CHERP;
ERPROT213-21) impairs Ca2+ mobilization, nuclear factor of activated
T-cells (NFAT) activation and cell proliferation in Jurkat
T-lymphocytes. Biochem J.;373.133-143.
LaPlante, J, O'Rourke F.,.Lu,X. Fein, A., Olsen, A and Feinsterin,
MB. (2000) Cloning of human Ca2+ homeostasis endoplasmic reticulum
protein (CHERP) inhibits intracellular Ca2+ mobilization and decreases
cell proliferation. Biochem. J. 348. 189-199.
Lu,X, Fein, A, Feinstein, M. and Orourke, F. (1999) Antisense
knockout of the Inositol 1,3,4,5-tetrakisphosphate receptor GAP1 IP4BP
in human erythroleukemia cell line leads to the appearance of
intermediate conductance K(Ca) channels that hyperpolarize the membrane
and enhance calcium influx.J. Gen. Physiol. 115, 81-95. |
