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Ingela Jansson
Assistant Professor of Cell Biology
ijansson@neuron.uchc.edu

• B.A., University of Stockholm
• Ph.D., University of Stockholm
• Cell Biology Graduate Program
• Not accepting students for Lab Rotations at this time

Dr. Jansson has two main areas of interest. Her major studies are on the cytochrome P450 (CYP)-containing monooxygenases responsible for drug, chemical and steroid oxidation. She has developed a heterologous expression system in Escherichia coli for the production of the different membranous forms of cytochrome P450 (Jansson, et al., "Enhanced expression of CYP1B1 in E. coli." Toxicology 144:211-219, 2000). She has also developed conditions for purification of these proteins and reconstitution systems to incorporated them into synthetic phospholipid vesicles with the reductase, NADPH-cytochrome P450 reductase in order to examine their monooxygenase characteristics. She applied her system to the study of involvement of CYP1B1 mutations in Primary Congenital Glaucoma (PCG), and was able to provide a possible explanation for the incomplete penetration in the disease phenotype of individuals with two different mutations in this hemoprotein. It had been shown that individuals with deletion genotypes of CYP1B1 developed PCG. In families with this disease individuals homozygous for the defective gene develop PCG. At least 26 mutations are known. A proportion of individuals homozygous for some of these point mutations did not develop PCG (incomplete penetrance). Dr. Jansson was able to show that two mutations, each resulting in a single amino acid substitution of CYP1B1, G61E and R469W, resulted in monooxygenases with considerably diminished activities (Jansson, et al.,"Effect of two mutations of human CYP1B1, G61E and R469W, on stability and endogenous steroid substrate metabolism." Pharmacogenetics 11:1-9 2001). She suggested that environmental factors could, in certain instances, cause elevations in the levels of these inducible enzymes, thus compensating for their diminished activities. This compensation might thus permit normal eye development.

A second area of Dr. Janssons studies have included examination of protein-protein interaction between cytochrome P450 forms and another membrane hemoprotein, cytochrome b₅. She has examined the nature of the charge pairing interaction between cytochrome b₅ and P450, and the topography of the enzymes within the endoplasmic reticulum membrane. Normally in a tissue, such as liver, there are at least a dozen different forms of cytochrome P450 present. Cytochrome b₅ can interact with a number of these forms. What determines whether such interaction occurs? Dr. Jansson was able to show that substrate is what stimulates this interaction (Jansson & Schenkman, "Substrate influence on interaction between cytochrome P450 and cytochrome b₅." Archives of Biochemistry and Biophysics 325:265-269, 1996). In further collaboration with Dr.Schenkman she was able to show the interaction was due to a charge-pairing involving basic residues on the proximal surface of cytochrome P450 and acidic residues around the heme edge of cytochrome b₅ (Schenkman, et al., "Charge-dependent sidedness of cytochrome p450 forms studied by quartz crystal microbalance and atomic force microscopy." Archives of Biochemistry and Biophysics 385:78-87, 2001). In a recent review current understanding of the interactions between these hemoproteins in the monooxygenase reaction is discussed (Schenkman & Jansson, "The many roles of cytochrome b₅" Pharmacology & Therapeutics 97:139-152, 2003).

Laboratory Home Page

Publications

Selected Publications:

Choudhary D, Jansson I, Schenkman JB, Sarfarazi M, Stoilov I. Comparative expression profiling of 40 mouse cytochrome P450 genes in embryonic and adult tissues. Arch Biochem Biophys. 2003 Jun 1;414(1):91-100.

Estavillo C, Lu Z, Jansson I, Schenkman JB, Rusling JF. Epoxidation of styrene by human cyt P450 1A2 by thin film electrolysis andperoxide activation compared to solution reactions.

Biophys Chem. 2003 May 1;104(1):291-6.

Schenkman JB, Jansson I. The many roles of cytochrome b5. Pharmacol Ther. 2003 Feb;97(2):139-52. Review.

Bengtsson M, Danielsson F, Jansson IE, Johansson U. Identification of a new HLA-DPB1 allele,HLA-DPB1*9001. Tissue Antigens. 2002 Apr;59(4):344-6.

Hanas R, Adolfsson P, Elfvin-Akesson K, Hammaren L, Ilvered R, Jansson I, Johansson C, Kroon M, Lindgren J, Lindh A, Ludvigsson J, Sigstrom L, Wiik A, Aman J, Jansson I, Wilk A. Indwelling catheters used from the onset of diabetes decrease injection pain and pre-injection anxiety. J Pediatr. 2002 Mar;140(3):315-20. Erratum in: J Pediatr 2002 Aug;141(2):298.