|
Faculty Marja M. Hurley
Professor of Medicine
hurley@nso1.uchc.edu
Areas of Interest:
My laboratory examines molecular mechanisms by which members of the
fibroblast growth factor (FGFs) and fibroblast growth factor receptor (FGFR)
families, (produced by osteoblasts, osteoclasts and stromal cells)
regulate bone development, remodeling and disorders of bone. Fgf2
knockout and Fgf2 transgenic mice are utilized in loss and gain of
function experiments to elucidate the role of FGF-2 in disorders of bone
including osteoporosis. Other projects involve examination of the
effects of cytokines and hormones including interleukin-1 (IL-1),
parathyroid hormone (PTH), insulin-like growth factor-I (IGF-I),
transforming growth factor beta (TGFb), prostaglandins (PGs) and
glucocorticoids on FGF2 and FGFR signaling, gene transcription, mRNA
stability and the activity of transfected Fgf2 promoter-luciferase
reporter gene constructs.
Lab Rotation Projects:
Areas of interest; Molecular mechanisms by which members of the
fibroblast growth factor (FGFs) and fibroblast growth factor receptor (FGFR)
families, (produced by osteoblasts, osteoclasts and stromal cells)
regulate bone development, remodeling and disorders of bone.
Project 1- Fgf2 knockout and Fgf2 transgenic mice are utilized in
loss and gain of function experiments to elucidate the role of FGF-2 in
disorders of bone including osteoporosis.
Project 2- Examination of the effects of cytokines and hormones
including interleukin-1 (IL-1), parathyroid hormone (PTH), insulin-like
growth factor-I (IGF-I), transforming growth factor beta (TGF),
prostaglandins (PGs) and glucocorticoids on FGF2 and FGFR signaling,
gene transcription, mRNA stability and the activity of transfected Fgf2
promoter-luciferase reporter gene constructs.
Selected Publications:
Sabbieti MG, Marchetti LM, Gabrielli MG, Menghi M, Materazzi S,
Menghi G, Raisz LG, Hurley MM (2005) Prostaglandins differently regulate
FGF-2 and FGF receptor expression and induce nuclear translocation in
osteoblasts via MAPK Kinase. Cell and Tissue Research 319:267-278
Sobue T, Naganawa T, Xiao L, Okada Y, Tanaka Y, Ito M, Okimoto N,
Nakamura T, Coffin JD, Hurley MM (2005). Overexpressing Fibroblast
Growth Factor-2 Causes Defective Bone Mineralization and Osteopenia in
Transgenic Mice. Journal of Cellular Biochemistry 95:83-94
Xiao L, Naganawa T, Obugunde E, Gronowicz G, Ornitz DM, Coffin JD,
and Hurley MM. (2004) Stat1 controls post natal bone formation by regulating FGF signaling in osteoblasts
J. Biol. Chem, 279, 27743-27751.
Kalajzic I, Kalajzic Z, Hurley MM, Lichtler A.C. Rowe DW. (2003)
Stage Specific Inhibition of Osteoblast Lineage Differentiation by FGF-2
and Noggin J Cell Biochem, 88:1168-1176
Okada Y, Montero A, Zhang X, Sobue T, Lorenzo J, Doetschman T, Coffin
JD, Hurley MM (2003) Impaired osteoclast formation in bone marrow
cultures of Fgf2 null mice in response to parathyroid hormone. J Biol
Chem 278:21258-21266
Xiao L, Liu P, Sobue T, Lichtler A, Coffin JD, Hurley MM (2003)
Effect of overexpressing fibroblast growth factor 2 protein isoforms in
osteoblastic ROS 17/2.8 cells. Journal of Cellular Biochemistry
89:1291-130
Sobue T, Gravely T, Hand A, Min YK, Pilbeam C, Raisz LG, Zhang, X,
Larocca D, Florkiewicz R, Hurley MM. (2002) Regulation of fibroblast
growth factor-2 and fibroblast growth factor receptors by transforming
growth factor beta in human osteoblastic MG-63 Cells. J. Bone Miner Res,
17:502-512.
Menghi G, Sabbieti MG, Marchetti L, Menghi M, Materazzi S, Hurley MM.
(2002) Pthalate Esters Influence FGF-2 Translocation in Py1a Rat
Osteoblasts. European Journal of Morphology 39:10-18.
Hurley MM, Marie PJ., Florkiewicz R. (2002) Fibroblast Growth Factor
and Fibroblast Growth Factor Receptor Families in Bone. In Principles of
Bone Biology, ed. by J. Bilezikian, L.G. Raisz and G. Rodan. Academic
Press pp 825-851.
Zhang X, Sobue T, Hurley MM. (2002) FGF-2 Increases Colony Formation,
PTH receptor, and IGF-1 mRNA in mouse Marrow Stromal Cells. Biochem,
Biophys. Res. Comm, 290:526-531.
Sobue T, Zhang X, Florkiewicz RZ, Hurley MM. (2001) Interleukin-1
Regulates FGF2 mRNA and localization of FGF-2 protein in human
osteoblasts. Biochem, Biophys. Res. Comm, 286:33-40.
|
