|
Faculty David Han
Assistant Professor of Physiology
han@nso.uchc.edu
Areas of Interest:
We are interested in signal transduction pathways controlled by
protein complexes. Multiple signal transduction pathways are controlled
by protein complexes that collaborate together and examples of the
protein complexes have been described in cell proliferation, cell
migration, differentiation, as well as apoptosis signaling. We utilize
standard biochemical techniques such as protein fractionation, as well
newer technologies such as nano-electrospray liquid chromatography
tandem mass spectrometry to study protein complexes, their identities,
abundance, and activities. Biological information that we experimentally
measured are stored in an interactive database where a number of
information processing and modeling are further explored.
Lab Rotation Projects:
Proteomics and Protein: Protein Interaction Networks
Students who wish to understand the basic techniques related to
proteomics and want to learn how to use proteomics to understand
biologically relevant protein : protein interaction networks can
participate in a rotation project.
#1 – Annexin I interacting proteins: We have recently showed that
Annexin I protein is crucial for the recognition and engulfment of
apoptotic cells. We have a good antibody that can be used to
immunoprecipitate Annexin I and associated protein complexes. Students
will participate in immunoprecipitation of endogenous Annexin I from
viable cells or apoptotic cells to identify the proteins that
co-precipitate with annexin I. Students are anticipated to learn basic
protein identification technique using the liquid-chromatography and
tandem mass spectrometry.
#2 - Phosphotidylserine Receptor interacting protein networks: We
have also showed that Phosphatidylserine Receptor (PSR) participates in
the engulfment of apoptotic cells by recognizing cell-surface exposed
annexin I. Thus, we are interested in identifying PSR associated
proteins during the engulfment of apoptotic cells. Students will
participate in PSR immunoprecipitation experiments and identification of
cell surface receptors and intracellular signaling proteins.
#3- Cancer Biology Projects: We are interested in identifying
cancer specific oncoproteins / tumor suppressor protein from
histological tissue arrays. We have recently developed methods to
dientify proteins directly from human prostate cancer tissues. Students
will participate in optimizing the protein extraction conditions to
detect low quantity of proteins from tumor cells using very sensitive
mass spectrometry and proteomics technologies. At the end of the
rotation, students are expected to learn a number of techniques related
to proteomics and cancer biology.
Selected Publications:
Taylor CM, Marta CB, Claycomb RJ, Han DK, Rasband MN, Coetzee T,
Pfeiffer SE. 2004. Proteomic mapping provides powerful insights into
functional myelin biology. Proc Natl Acad Sci U S A. Mar
30;101(13):4643-8.
Chang SH, Liu CH, Conway R, Han DK, Nithipatikom K, Trifan OC, Lane
TF, Hla T. 2004. Role of prostaglandin E2-dependent angiogenic switch in
cyclooxygenase 2-induced breast cancer progression. Proc Natl Acad Sci U
S A. Jan 13;101(2):591-6.
Lundgren DH, Eng J, Wright ME, Han DK. 2003. PROTEOME-3D: An
Interactive Bioinformatics Tool for Large-Scale Data Exploration and
Knowledge Discovery. Mol Cell Proteomics. Nov;2(11):1164-76. Epub 2003
Sep 07.
Arur S, Uche UE, Rezaul K, Fong M, Scranton V, Cowan AE, Mohler W,
Han DK. 2003. Annexin I is an endogenous ligand that mediates apoptotic
cell engulfment. Dev Cell. Apr;4(4):587-98.
Imanishi T, Han DK, Hofstra L, Hano T, Nishio I, Liles WC, Gown AM,
Schwartz SM, Han DK, Gorden AM. 2002. Apoptosis of vascular smooth
muscle cells is induced by Fas ligand derived from monocytes/macrophage.
Atherosclerosis. Mar;161(1):143-51. Erratum in: Atherosclerosis.
Mar;167(1):175.. Han David KM [corrected to Han David K]; Gorden Alan M
[corrected to Gown Alan M].
Griffin TJ, Han DK, Gygi SP, Rist B, Lee H, Aebersold R, Parker KC.
2001. Toward a high-throughput approach to quantitative proteomic
analysis:expression-dependent protein identification by mass
spectrometry. J Am Soc Mass Spectrom. Dec;12(12):1238-46.
Han DK, Eng J, Zhou H, Aebersold R. 2001. Quantitative profiling of
differentiation-induced microsomal proteins using isotope-coded affinity
tags and mass spectrometry. Nat Biotechnol. Oct;19(10):946-51.
Imanishi T, Hano T, Nishio I, Han DK, Schwartz SM, Karsan A. 2001.
Apoptosis of vascular smooth muscle cells is induced by Fas ligand
derived from endothelial cells. Jpn Circ J. Jun;65(6):556-60. Erratum
in: Jpn Circ J 2002 Dec;66(12):1185.
Han MH, Han DK, Aebersold RH, Glomset JA. 2001. Effects of protein
kinase CK2, extracellular signal-regulated kinase 2, and protein
phosphatase 2A on a phosphatidic acid-preferring phospholipase A1. J
Biol Chem. Jul 20;276(29):27698-708. |
