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Faculty
 Gordon G. Carmichael
Professor of Genetics & Developmental Biology
gcarmich@neuron.uchc.edu
Areas of Interest:
The primary interests of my group are the molecular signals which
control the expression and function of mRNA molecules. We study RNA
polymerase II promoters and the mechanisms of splicing, polyadenylation
and RNA export from the nucleus to the cytoplasm. A recent area of
interest has been the mechanism of action of naturally-occurring
antisense RNA regulation in mammalian cells. Our preferred model system
is the mouse polyoma virus, but we are also using other systems,
including histone genes, the HIV-1 virus and yeast.
Lab Rotation Projects:
We study the consequences of double strand RNA in cells, especially with
regard to what happens in the nucleus. Our work includes studies on RNA
editing, the nucleocytoplasmic transport of RNA molecules, and
mechanisms by which dsRNAs can lead to gene silencing. Rotation projects
would most likely involve molecular studies on proteins we have
identified that play important roles in dsRNA metabolism.
Selected Publications:
Q. Wang and G.G. Carmichael: RNA Silencing. The Biologist, in press.
Q. Wang and G.G. Carmichael: Effects of length and location on the
fate of dsRNA. Microbiol. Molec. Biol. Rev., in press.
Z. Zhang and G.G. Carmichael: Methods for the analysis of
adenosine-to-inosine in RNA (2004) Methods in Molec. Biol., 257: 75-83.
G.G. Carmichael: Antisense starts making more sense (2003) Nature
Biotechnology 21: 371-372.
Z. Zhang and G.G. Carmichael: Mechanism of action of antisense RNA in
eukaryotic cells. (2003) In, “Cancer Drug Discovery and development:
Nucleic Acid Therapeutics in Cancer”, (A. Gewirtz, ed.), Humana Press,
Totowa, NJ, pp. 89-104.
G.G. Carmichael: Silencing viruses with RNA. (2002) Nature 418:
379-380.
Y. Huang and G.G. Carmichael: "Nucleocytoplasmic mRNA transport".
(2001) In, Results and Problems in Cell Differentiation, (D. Richter,
ed.) vol. 34, pp. 140-155, Springer-Verlag, Berlin.
L.E. Maquat and G.G. Carmichael: Quality control of mRNA function.
(2001), Cell 104: 173-176.
Z. Zhang and G.G. Carmichael: The fate of double-stranded RNA in the
nucleus: A multiprotein complex containing the inosine-specific
RNA-binding protein p54nrb mediates the nuclear retention of A-to-I
edited RNAs. (2001) Cell 106: 465-475. |
