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Faculty Paul Campagnola
Assistant Professor of Cell Biology
campagno@neuron.uchc.edu
Areas of Interest:
Second Harmonic Generation (SHG) Imaging studies of fibrous
structural proteins including collagen, keratin, and myosin. Aspects
include studying macromolecular, long -range order on the micron scale
as well as quantifying the non-linear optical properties. The emphasis
is on using SHG as a diagnostic for collagen and amyloid related
diseases as well as possible photonic device construction from these
materials.
Micro and nanofabrication of biologically relevant materials using
multi-photon excitation. Applications are in the areas of tissue
engineering, microfluidics, sustained release and biosensing.
Development and optimization of new photochemistries, and physical and
optical characterization of fabricated structures. Implementation of new
non-linear optical schemes to produce minimum feature sizes in the
50-100 nm range.
Photophysics of gold and silver nanoparticles linked to environmentally
sensitive dyes. The origin of the non-linear optical properties are
investigated by high-resolution SHG and multi-photon excited
fluorescence imaging by varying colloid size, excitation wavelength,
chromophore, and by polarization anisotropy analysis. A goal of this
work is the application of these particles as chemical sensors in
biological systems.
Development of novel nonlinear microscopes and methods for imaging cells
and cellular membranes including two and three photon excited
fluorescence, multi-photon fluorescence lifetime imaging, and
multi-photon photoactivation/photobleaching, second harmonic generation
and third harmonic generation in both wide-field and near-field
configurations.
Selected Publications:
S. Basu and P.J. Campagnola, “Enzymatic Activity of Alkaline
Phosphatase inside Protein and Polymer Structures Fabricated via
Multi-photon Excitation” Biomacromolecules, in press
S. Basu, C.W. Wolgemuth, and P.J. Campagnola, “Measurement of
Normal and Anomalous Diffusion of Dyes within Protein Structures
Fabricated via Multi-photon Excited Crosslinking,” J. Phys. Chem B,
submitted.
A.C. Millard, P.J. Campagnola, W.A. Mohler, A. Lewis, and L.M. Loew,
“Second Harmonic Imaging Microscopy” Methods in Enzymology, 361, 47-69
(2003).
P.J. Campagnola, A.C. Millard, and W.A. Mohler, “Second Harmonic
Generation Imaging Microscopy of Endogenous Structural Proteins”
Methods, 29, 97 – 109 (2003).
William A. Mohler and P.J. Campagnola, “Nonlinear optical spectroscopy
and imaging of structural proteins in living tissues” Optics and
Photonics News, 14, 40-45 (2003).
R.M. Brown, Jr, A.C. Millard, and P.J. Campagnola, “Macromolecular
Structure of Cellulose Studied by Second Harmonic Generation Imaging
Microscopy” Optic Lett,22, 2207-2209 (2003).
L.M. Loew and P.J. Campagnola, “Exact change required: Second
harmonic imaging microscopy visualizes distinct biomolecular arrays in
live cells tissues and organisms” Nature Biotech, 11, 1356-1360, (2003).
L.M. Loew, P.J. Campagnola, A. Lewis, and J.P. Wuskell, “Confocal and
Non-linear Optical Imaging of Potentiometric Dyes” Methods in Cell
Biology, 70, 429-453 (2002).
Paul J. Campagnola, Mark Terasaki, P.E. Hoppe, C.J. Malone, and W. A.
Mohler, "3-Dimesional High-Resolution Second Harmonic Generation Imaging
of Endogenous Structural Proteins in Biological Tissues" Biophys J., 81,
493-508(2002).
J. D. Pitts, A. R. Howell, I. Banerjee, J. Wang, S. L. Goodman, and P.
J. Campagnola, ” New photoactivators for multi-photon excited 3
dimensional sub-micron crosslinking of proteins.” Photochem Photobiol,
76 135-144 (2002).
P. J. Campagnola, Heather A. Clark, William A. Mohler, Aaron Lewis,
and Leslie M. Loew, "Second Harmonic Imaging Microscopy of Living Cells"
J. Biomedical Optics, 6, 277-286 (2001).
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