Areas of Interest
The major interest of our laboratory has been to elucidate the
mechanisms by which hormones and growth factors regulate bone
remodeling in postnatal life, with an emphasis on the
development and characterization of transgenic and mutant mouse
models. Our current projects include investigating the roles of
insulin-like growth factor and glucocorticoids on bone
remodeling and examining the role that a dominant negative
member of the CREM transcription factor family plays in
parathyroid hormone-dependent gene expression in osteoblasts.
Lab Rotation Projects
The current research projects in the laboratory involve using
transgenic and knockout mouse models to understanding the role
of hormones and growth factors in bone biology. The specific
areas of interest include the role of insulin-like growth
factor-I in bone development and remodeling, molecular
mechanisms by which endogenous glucocorticoids promote bone
mineralization and the role of ATF/CREB transcription factors in
Lengner CJ, Steinman HA, Gagnon J, Kream BE, Stein GS, Lian JB,
Jones SN 2006 Osteoblast differentiation and skeletal
development are regulated by Mdm2-p53 signaling. J Cell Biol.
He J, Rosen CJ, Adams DJ, Kream BE 2006 Postnatal growth and
bone mass in mice with IGF-I haploinsufficieny. Bone 38:826-35.
Jiang J, Lichtler AC, Gronowicz GA, Adams DJ, Clark SH, Rosen
CJ, Kream BE 2006 Transgenic mice with osteoblast-targeted
insulin-like growth facto-I show increased bone remodeling. Bone
Epub, April 24.
Liu F and Kream BE Identification of novel Crem isoforms
expressed by osteoblasts 2005 Calcif Tiss Int. 77:91-95.
Liu F, Woitge HW, Braut A, Kronenberg MS, Lichtler AC, Mina
M, Kream BE 2004 Expression and activity of osteoblast-targeted
Cre recombinase transgenes in murine skeletal tissues. Int J Dev
Sher LB, Woitge HW, Adams DJ, Krozowski Z, Harrison JR and
Kream BE 2004 Transgenic expression of 11ß-hydroxysteroid
dehydrogenase type 2 in osteoblasts reveals an anabolic role for
endogenous glucocorticoids in bone. Endocrinology 145:922-929.
Nervina JM, Tetradis S, Huang, Y, Harrison D, Molina C and
Kream BE 2003 Expression of inducible cAMP early repressor is
coupled to the cAMP-protein kinase A signaling pathway in
osteoblasts. Bone 32:483-490.
Harrison JR, Woitge HW and Kream BE 2002 Genetic approaches
to determine the role of glucocorticoid signaling in osteoblasts.
Woitge HW, Harrison JR, Ivkosic A, Krozowski Z and Kream BE
2001 Cloning and in vitro characterization of
Col1a1-11ß-hydroxysteroid dehydrogenase type 2 transgenes as
models for osteoblast-selective inactivation of natural
glucocorticoids. Endocrinology 142:1341-1348.