Areas of Interest
Dyneins are microtubule-based molecular motors that power both
ciliary/flagellar motility and a variety of essential
intracellular motile events. In order for these massive enzymes
to function correctly, they must be attached to the appropriate
cargo and motor activity must be precisely regulated. Over the
last few years we have identified a series of highly intriguing
dynein components that appear to be involved in these regulatory
activities. Current focus in the laboratory is on understanding
the mechanisms which control motor function using a wide variety
of techniques ranging from physiological measurements and
genetic analyses to structural biology.
Lab Rotation Projects
1) Examination of dynein components as candidates for high
resolution structural analysis.
2) Genetic screen for mutants defective in flagellar motility.
3) Biochemical approaches to defining intra-dynein
associations.
4) Functional analysis of dyneins expressing mutant components.
5) Function of flagellar-associated nucleoside diphosphate
kinases.
6) Chlamydomonas metabolomics using NMR and/or mass
spectrometry.
Selected Publications
Wanschers B, van de Vorstenbosch R, Wijers M, Wieringa B, King
SM, Fransen J. Rab6 family proteins interact with the dynein
light chain protein DYNLRB1. Cell Motil Cytoskeleton. 2008
Mar;65(3):183-96.
Lo KW, Kogoy JM, Rasoul BA, King SM, Pfister KK. Interaction
of the DYNLT (TCTEX1/RP3) light chains and the intermediate
chains reveals novel intersubunit regulation during assembly of
the dynein complex. J Biol Chem. 2007 Dec 21;282(51):36871-8.
Epub 2007 Oct 27.
Wakabayashi K, Sakato M, King SM. Protein modification to
probe intradynein interactions and in vivo redox state. Methods
Mol Biol. 2007;392:71-83.
Rompolas P, Pedersen LB, Patel-King RS, King SM.
Chlamydomonas FAP133 is a dynein intermediate chain associated
with the retrograde intraflagellar transport motor. J Cell Sci.
2007 Oct 15;120(Pt 20):3653-65.
Sakato M, Sakakibara H, King SM. Chlamydomonas outer arm
dynein alters conformation in response to Ca2+. Mol Biol Cell.
2007 Sep;18(9):3620-34.
Pedersen LB, Rompolas P, Christensen ST, Rosenbaum JL, King
SM.
The lissencephaly protein Lis1 is present in motile mammalian
cilia and requires outer arm dynein for targeting to
Chlamydomonas flagella.
J Cell Sci. 2007 Mar 1;120(Pt 5):858-67.
Hozumi A, Satouh Y, Makino Y, Toda T, Ide H, Ogawa K, King
SM, Inaba K.
Molecular characterization of Ciona sperm outer arm dynein
reveals multiple components related to outer arm docking complex
protein 2.
Cell Motil Cytoskeleton. 2006 Oct;63(10):591-603.
Wakabayashi K, King SM. Modulation of Chlamydomonas
reinhardtii flagellar motility by redox poise. J Cell Biol. 2006
Jun 5;173(5):743-54.
Yang P, Diener DR, Yang C, Kohno T, Pazour GJ, Dienes JM,
Agrin NS, King SM, Sale WS, Kamiya R, Rosenbaum JL, Witman GB.
Radial spoke proteins of Chlamydomonas flagella. J Cell Sci.
2006 Mar 15;119(Pt 6):1165-74.
Pfister KK, Shah PR, Hummerich H, Russ A, Cotton J, Annuar
AA, King SM, Fisher EM. Genetic analysis of the cytoplasmic
dynein subunit families.
PLoS Genet. 2006 Jan;2(1):e1. |
