Areas of Interest
I have been using advanced light microscopy techniques to
investigate intracellular organization and function. Most of my
work has involved imaging of living cells using confocal
microscopy. I have recently completed work using green
fluorescent protein chimeras to label the ER and Golgi apparatus
during the cell cycle in sea urchin embryos. Three current
projects are: how cells heal wounds in the plasma membrane;
changes in ER structure during meiotic maturation; and nuclear
envelope breakdown, where we are planning to use some computer
modeling and two photon microscopy to understand steps involved
in this process.
Lab Rotation Projects
Terasaki/Fein Lab
Cell Wounding Project
We have developed an electrophysiological method for studying
plasma membrane wound repair in single cells. Large plasma
membrane disruptions in starfish oocytes, made by femtosecond
pulses from a Ti-Sapphire laser, are repaired within a few
seconds as shown by stabilization of membrane electrical
properties and restoration of dye exclusion. The membrane
potential after wounding is sensitive to the extracellular Cl-
concentration but not to that of Na+, K+ or H+ indicating that
Cl- permeable intracellular membranes have fused with the plasma
membrane. We believe that cell wounding provides a novel means
for the electrophysiological analysis of chloride permeable
intracellular membranes that have been translocated to the
plasma membrane.
Selected Publications
Terasaki, M. 2006. Fluorescence quantitation in thick
specimens, with an application to cyclin B-GFP expression in
starfish oocytes. Biol. Cell (in press).
Fein, A., and Terasaki, M. 2005. Rapid increase in plasma
membrane chloride permeability during wound resealing in
starfish oocytes. J. Gen. Physiol. 126: 151-159.
Millard, A.C., Terasaki, M., and Loew, L.M. 2005. Second
harmonic imaging of exocytosis at fertilisation. Biophys. J.
Biophys J 88: L46-48.
Lénárt, P., Daigle, N., Bacher, C., Hand, A.R., Eils, R.,
Terasaki, M., and Ellenberg, J. 2005. A contractile nuclear
actin network drives chromosome congression in oocytes. Nature
436: 812-818.
Terasaki, M., Okumura, E., Hinkle, B., and Kishimoto, T.
2003. Localization and dynamics of Cdc2-cyclin B during meiotic
reinitation in starfish oocytes. Mol. Biol. Cell 14:4685-4694.
Slepchenko, B.M., and Terasaki, M. 2003. Cyclin aggregation
and robustness of bio-switching. Mol. Biol. Cell 14: 4695-4706.
Galbraith, J.A., and Terasaki, M. 2003. Controlled damage in
thick specimens by multiphoton excitation. Mol. Biol. Cell 14:
1808-1817.
Hinkle, B., Slepchenko, B., Rolls, M.M., Walther, T.C.,
Stein, P.A., Mehlmann, L.M., Ellenberg, J., and Terasaki, M.
2002. Chromosomal association of Ran during meiotic and mitotic
divisions. J. Cell Sci. 115: 4685-4693.
Terasaki, M., Runft, L., and Hand, A. 2001. Organization of
the endoplasmic reticulum during Xenopus oocyte maturation and
egg activation. Mol. Biol. Cell 12: 1103-1116.
Terasaki, M., Campagnola, P., Rolls, M.M., Stein, P.,
Ellenberg, J., Hinkle, B., and Slepchenko, B. 2001. A new model
for nuclear envelope breakdown. Mol. Biol. Cell 12: 503-510. |
