Research Interest
We are interested in the molecular mechanisms that regulate
tumor progression in the colon. Our work encompasses the study
of signaling pathways both in mouse tumor models as well as in
immortalized cell lines. A major focus of our research is to use
genome-wide profiling to identify unique patterns of expression
and to discover new genes that predict tumor progression and
metastasis.
Lab Rotation Projects:
1. Role of intra- and extracellular phospholipases in
the control of tumorigenesis. Studies on the effects of genetic
deletion of cytoplasmic phospholipase A2 in apoptosis and
carcinogenesis initiated by exposure to tumor initiating events.
2. Gene profiling of adenomas as a means to establish
efficacy of chemoprevention agents in inhibiting cancer cell
growth.
3. Genome-wide analysis of chromosomal gains/losses in
early precancerous lesions. Identification of gene polymorphisms
as predisposing factors to cancer risk.
4. Functional analyses of potential tumor suppressors
in tumor cell culture systems.
5. Inflammation driven cancer in the intestine.
6. Mechanisms of tumor cell invasion and metastasis
using RNAi and overexpression of candidate genes.
7. Analysis of the p53-ARF-mdm2 oncogene checkpoint in
the control of tumorigenesis. Analysis of mdm-2 status in tumor
cells.
Selected Publications
Greenspan EJ, Cyr JL, Pleau DC, Levine J, Rajan TV, Rosenberg
DW and Heinen CD. 2006. Microsatellite instability in aberrant
crypt foci from patients without concurrent colon cancer
(Carcinogenesis, in press).
Stevens, RG, Swede, H and Rosenberg, DW. 2006. Epidemiology
of colonic aberrant crypt foci: Review and analysis of existing
studies (Cancer Letters, in press).
Greenspan EJ, Jablonski M, Rajan TV, Levine J, Belinsky GS
and Rosenberg DW. 2006. Epigenetic alterations in RASSF1A in
human aberrant crypt foci. Carcinogenesis 27:1316-22.
Belinsky, GS, Rajan, TV, Saria, E and Rosenberg, DW. 2006.
Expression of secretory phospholipase A2 in colon tumor cells
potentiates tumor growth (Molecular Carcinogenesis, in press).
Flynn, C, Montrose, DC, Swank, DL, Nakanishi, M, Ilsley, JNM
and Rosenberg DW. 2006. Deoxycholic acid promotes the growth of
colonic aberrant crypt foci (Molecular Carcinogenesis, in
press).
Mann JR, Backlund MG, Buchanan FG, Daikoku T, Holla VR,
Rosenberg DW, Dey SK and DuBois RN. 2006. Repression of
Prostaglandin Dehydrogenase by EGF and Snail Increases
Prostaglandin E-2 and Promotes Cancer Progression. Cancer
Research, 66:6649-6656.
Aizu W, Belinsky GS, Flynn C, Noonan EJ, Boes C, Godman CA,
Nambiar PR, Rosenberg DW and Gardina C. 2006. Circumvention and
reactivation of the p53 oncogene checkpoint in mouse colon
tumors Biochemical Pharmacology, 72(8):981-91.
Nakanishi, M and Rosenberg, DW. 2006. Roles of CPLA2-alpha
and arachidonic acid in cancer (review article) Biochem Biophys
ACTA, 1761:1335-43.
Stevens RG, Swede, H, Jablonski M, Parente M, Tirnauer JS,
Heinen CD, Giardina C, Rajan TV, Rosenberg DW and Levine J.
2006. Aberrant Crypt Foci in Persons with a Family History of
Colorectal Cancer (Cancer Letters, Epub, in press).
Kohno M, Momoi M, Oo ML, Paik J-H, Lee Y-M, Venkataraman K,
Xi Y, Ristimaki AP, Fyrst H, Sano H, Rosenberg DW, Saba JD,
Proia RL, Hla T. 2006. An intracellular role for Sphingosine
Kinase 1 in intestinal adenoma cell proliferation. (Molecular
Cellular Biology, 26:7211-23.
Belinsky GS, Claffey KP, Nambiar PR, Guda K, Rosenberg DW.
2005. Vascular endothelial growth factor and enhanced
angiogenesis do not promote metastatic conversion of a newly
established azoxymethane-induced colon cancer cell line.
Molecular Carcinogenesis 43(2):65-74.
Tong X, Yin L, Joshi S, Rosenberg DW, Giardina C. 2005.
Cyclooxygenase-2 regulation in colon cancer cells: modulation of
RNA polymerase II elongation by HDAC inhibitors. J Biol Chem
22:15503-15509.
Nambiar PR, Boutin, SR, Raja R, Rosenberg DW. 2005. Global
gene expression profiling: a complement to conventional
histopathological analysis of neoplasia. Veterinary Pathology
42:735-752. |
