Areas of Interest
My laboratory’s research interests include development of
autonomic innervation and of autonomic neuroeffector
transmission in the embryonic chick heart and
excitation-contraction coupling in mammalian heart cells and its
regulation by autonomic neurotransmitters. These themes require
evaluation of signal transduction pathways in single ventricular
myocytes by simultaneous measurements of ionic currents,
intracellular calcium transients and contractions. Ionic
currents are measured under voltage clamp conditions with the
whole cell patch electrode technique. Single cell contractions
are recorded with a sensitive video edge detector system.
Intracellular calcium transients are detected with the
fluorescent indicator, fura-2. The role of intracellular
messengers (cAMP, cGMP, Ins 1,4,5-P3) in the action
of neurotransmitters (acetylcholine, norepinephrine) is studied
by intracellular dialysis through the patch pipette.
Selected Publications
Gomez AM, Kerfant BG, Vassort G, Pappano AJ. 2004. Autonomic
regulation of calcium and potassium channels is oppositely
modulated by microtubules in cardiac myocytes. Am J Physiol (in
press).
Pappano, AJ and Katzung, BG. 2004. “Cholinoceptor-Blocking
Drugs” in Basic and Clinical Pharmacology (9th Edition),
Katzung, BG ed. MacGraw-Hill, New York. 109-121.
Pappano, AJ 2004. “Cholinoceptor-Activating and
Cholinesterase-Inhibiting Drugs” in Basic and Clinical
Pharmacology (9th Edition), Katzung, BG ed. MacGraw-Hill,
New York. 94-108.
Pappano, AJ, Imai, Y and Jiang, B. 2002. Reply to letter to
the editor. Cardiovasc. Res. 54:699-700.
Shen JB, Pappano AJ. 2002. On the role of phosphatase in
regulation of cardiac L-type calcium current by cyclic GMP. J
Pharmacol Exp Ther. May;301(2):501-6.
Imai Y, Jiang B, Pappano AJ. 2001. Mechanism for muscarinic
inhibition of I(Ca(L)) is determined by the path for elevating
cyclic AMP in cardiac myocytes. Cardiovasc Res. Aug
1;51(2):331-43.
Shen JB, and Pappano AJ. 2001. Carbachol inhibits the L-type
Ca2+ current augmented by
1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid in
guinea pig ventricular myocytes: calcium-sensitivity hypothesis
for muscarinic inhibition. J Pharmacol Exp Ther.
Aug;298(2):857-64.
Shen, J.-B., Jiang, B. and Pappano, A. J. 2000 A Comparison
of L-type Calcium Channel Blockade by Nifedipine and/or Cadmium
in Guinea Pig Ventricular Myocytes. J. Pharmacol. Exp. Ther.
294:562-570.
|
