Areas of Interest
Research is focused on signal transduction in human platelets
with specific interest in the inositol phosphate signaling
pathway and its regulation. The IP3 and IP4 receptor systems are
currently being investigated to include characterization and
purification using techniques of HPLC and affinity
chromatography. A HEL cell cDNA expression library using
monoclonal antibodies and oligonucleotide probes is also being
used to clone and sequence the gene for the receptors.
Selected Publications
O'Rourke FA, LaPlante JM, Feinstein MB. (2003) Antisense-mediated
loss of calcium homoeostasis endoplasmic reticulum protein (CHERP;
ERPROT213-21) impairs Ca2+ mobilization, nuclear factor of
activated T-cells (NFAT) activation and cell proliferation in
Jurkat T-lymphocytes. Biochem J.;373.133-143.
LaPlante, J, O'Rourke F.,.Lu,X. Fein, A., Olsen, A and
Feinsterin, MB. (2000) Cloning of human Ca2+ homeostasis
endoplasmic reticulum protein (CHERP) inhibits intracellular
Ca2+ mobilization and decreases cell proliferation. Biochem. J.
348. 189-199.
Lu,X, Fein, A, Feinstein, M. and Orourke, F. (1999) Antisense
knockout of the Inositol 1,3,4,5-tetrakisphosphate receptor GAP1
IP4BP in human erythroleukemia cell line leads to the appearance
of intermediate conductance K(Ca) channels that hyperpolarize
the membrane and enhance calcium influx.J. Gen. Physiol. 115,
81-95. |
