Areas of Interest
As the NMR Facility Manager I primarily utilize nuclear
magnetic resonance (NMR) to characterize the three dimensional
structure, function and dynamics of proteins from a wide range
of biological systems. Research in our laboratory has focused in
the past on enzymes involved in DNA repair, specifically the
base excision repair pathway. More recently several
collaborative projects have been initiated studying proteins
involved in bacterial cell division, neurite outgrowth, dynein
motor complexes, and others. Much future work involve studying
several insecticidal neurotoxins from the African funnel web
spider with potential to be used in insect control in
collaboration with Dr. Glenn King.
Selected Publications
Rowland SL, Burkholder WF, Cunningham KA, Maciejewski MW,
Grossman AD, King GF. Structure and mechanism of action of Sda,
an inhibitor of the histidine kinases that regulate initiation
of sporulation in Bacillus subtilis. Mol Cell. 2004,13:689-701.
Wu H, Blackledge M, Maciejewski MW, Mullen GP, King SM.
Relaxation-based structure refinement and backbone molecular
dynamics of the dynein motor domain-associated light chain.
Biochemistry. 2003, 42:57-71.
Gryk MR, Marintchev A, Maciejewski MW, Robertson A, Wilson SH,
Mullen GP. Mapping of the interaction interface of DNA
polymerase beta with XRCC1. Structure (Camb). Structure. 2002,
10:1709-20.
Maciejewski MW, Shin R, Pan B, Marintchev A, Denninger A,
Mullen MA, Chen K, Gryk MR, Mullen GP. Solution structure of a
viral DNA repair polymerase. Nat Struct Biol. 2001;8:936-41.
Maciejewski MW, Liu D, Prasad R, Wilson SH, Mullen GP.
Backbone dynamics and refined solution structure of the
N-terminal domain of DNA polymerase beta. Correlation with DNA
binding and dRP lyase activity. J Mol Biol. 2000;296:229-53. |
