Areas of Interest
Epigenetics including the control of gene expression in
embryonic carcinoma stem cells and genomic imprinting and its
association with neurogenetics disorders.
Lab Rotation Projects
Epigenetic effects of tobacco smoke on human development.
The goals of this project are to first examine whether loss
of alteration of methylation of insulin-like growth factor 2
(IGF2) is associated with reduced fetal/placental growth in
women who smoke. This project may help us better understand
at a molecular level how smoking causes low birth weight babies.
The goals of this study are based on the observation that
tobacco smoking can alter normal patterns of DNA methylation and
the knowledge that DNA methylation regulates IGF2 imprinting. We
propose to study whether fetal tissue (umbilical cord), placenta
and maternal blood of smokers and non-smokers show differences
in methylation at the IGF2/H19 locus. These novel investigations
will permit us to determine whether the increased risk of
adverse obstetrical outcomes as result of tobacco usage have an
epigenetic basis and may help explain the variation of fetal
growth among smokers and the link between polycyclic aromatic
hydrocarbons and birth weight. Second, we will identify genes
that show altered expression in placenta and umbilical cord
tissue from smokers versus that of non-smokers.
Selected Publications
Sotirova VN, Calciano MA, Krueger W and Lalande M (2006).
Inclusion of a matrix-attached region in a 7SK pol III vector
increases the efficiency of shRNA-mediated gene silencing in
embryonic carcinoma cells. Plasmid, 55: 216-26.
Landers M , Calciano MA, Colosi D, Glatt-Deeley H, Wagstaff J
and Lalande M (2005). Maternal disruption of Ube3a leads to
increased expression of Ube3a-ATS in trans. Nucleic Acids Res.,
33: 3976-84.
Le Meur E, Watrin F, Landers M, Sturny R, Lalande M and
Muscatelli F (2005). Dynamic developmental regulation of the
large non-coding RNA associated with the mouse 7C imprinted
chromosomal region. Dev. Biol., 286: 587-600.
Simon J, Wakimoto H, Fujita N, Lalande M, Barnard EA. Analysis
of the set of GABAA and Glycine receptor genes in the human
genome (submitted).
Landers M, Bancescu D, Le Meur E, Rougeulle C, Glatt-Deeley
H, Brannan C, Lalande M. Regulation of the large (~1,000 kb)
imprinted murine Ube3a antisense transcript by alternative exons
upstream of Snurf/Snrpn (sumitted).
Bancescu D, Glatt-Deeley H, Lalande M. Epigenetic activation
of the 5-hydroxytryptamine (serotonin) receptor 2C in embryonal
carcinoma cells is DNA replication-dependent. Exp Cell Res (In
press).
Lalande M. Imprints of disease at GNAS1. J. Clin. Invest.
2001; 107: 793-794.
Wolverton T, Lalande M. Identification and Characterization
of Three Members of a Novel Subclass of Protocadherins. Genomics
2001; Vol. 76, Numbers 1-3, 66-72.
LaSalle J, Lalande M. Flow Cytometry and FISH to Investigate
Allele-Specific Replication Timing and Homologous Association of
Imprinted Chromosomes. Methods Mol Biol. 2001; 181:181-92
Cavaillé J, Buiting K, Kiefmann M, Lalande M, Brannan C,
Horsthemke B, Bachellerie JP, Brosius J and Hüttenhofer A.
Identification of imprinted tissue-specific C/D box small
nucleolar RNA genes in the Prader- Willi syndrome region. Proc.
Natl. Acad. Sci. USA 2000; 97: 14311-14316.
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