Areas of Interest
My laboratory examines molecular mechanisms by which members of
the fibroblast growth factor (FGFs) and fibroblast growth factor
receptor (FGFR) families, (produced by osteoblasts, osteoclasts
and stromal cells) regulate bone development, remodeling and
disorders of bone. Fgf2 knockout and Fgf2 transgenic mice are
utilized in loss and gain of function experiments to elucidate
the role of FGF-2 in disorders of bone including osteoporosis.
Other projects involve examination of the effects of cytokines
and hormones including interleukin-1 (IL-1), parathyroid hormone
(PTH), insulin-like growth factor-I (IGF-I), transforming growth
factor beta (TGFb), prostaglandins (PGs) and glucocorticoids on
FGF2 and FGFR signaling, gene transcription, mRNA stability and
the activity of transfected Fgf2 promoter-luciferase reporter
gene constructs.
Lab Rotation Projects
Areas of interest; Molecular mechanisms by which members of
the fibroblast growth factor (FGFs) and fibroblast growth factor
receptor (FGFR) families, (produced by osteoblasts, osteoclasts
and stromal cells) regulate bone development, remodeling and
disorders of bone.
Project 1- Fgf2 knockout and Fgf2 transgenic mice are
utilized in loss and gain of function experiments to elucidate
the role of FGF-2 in disorders of bone including osteoporosis.
Project 2- Examination of the effects of cytokines and
hormones including interleukin-1 (IL-1), parathyroid hormone (PTH),
insulin-like growth factor-I (IGF-I), transforming growth factor
beta (TGF), prostaglandins (PGs) and glucocorticoids on FGF2
and FGFR signaling, gene transcription, mRNA stability and the
activity of transfected Fgf2 promoter-luciferase reporter gene
constructs.
Selected Publications
Sabbieti MG, Marchetti LM, Gabrielli MG, Menghi M, Materazzi S,
Menghi G, Raisz LG, Hurley MM (2005) Prostaglandins differently
regulate FGF-2 and FGF receptor expression and induce nuclear
translocation in osteoblasts via MAPK Kinase. Cell and Tissue
Research 319:267-278
Sobue T, Naganawa T, Xiao L, Okada Y, Tanaka Y, Ito M,
Okimoto N, Nakamura T, Coffin JD, Hurley MM (2005).
Overexpressing Fibroblast Growth Factor-2 Causes Defective Bone
Mineralization and Osteopenia in Transgenic Mice. Journal of
Cellular Biochemistry 95:83-94
Xiao L, Naganawa T, Obugunde E, Gronowicz G, Ornitz DM,
Coffin JD, and Hurley MM. (2004) Stat1 controls post natal bone
formation by regulating FGF signaling in osteoblasts J. Biol.
Chem, 279, 27743-27751.
Kalajzic I, Kalajzic Z, Hurley MM, Lichtler A.C. Rowe DW.
(2003) Stage Specific Inhibition of Osteoblast Lineage
Differentiation by FGF-2 and Noggin J Cell Biochem, 88:1168-1176
Okada Y, Montero A, Zhang X, Sobue T, Lorenzo J, Doetschman
T, Coffin JD, Hurley MM (2003) Impaired osteoclast formation in
bone marrow cultures of Fgf2 null mice in response to
parathyroid hormone. J Biol Chem 278:21258-21266
Xiao L, Liu P, Sobue T, Lichtler A, Coffin JD, Hurley MM
(2003) Effect of overexpressing fibroblast growth factor 2
protein isoforms in osteoblastic ROS 17/2.8 cells. Journal of
Cellular Biochemistry 89:1291-130
Sobue T, Gravely T, Hand A, Min YK, Pilbeam C, Raisz LG,
Zhang, X, Larocca D, Florkiewicz R, Hurley MM. (2002) Regulation
of fibroblast growth factor-2 and fibroblast growth factor
receptors by transforming growth factor beta in human
osteoblastic MG-63 Cells. J. Bone Miner Res, 17:502-512.
Menghi G, Sabbieti MG, Marchetti L, Menghi M, Materazzi S,
Hurley MM. (2002) Pthalate Esters Influence FGF-2 Translocation
in Py1a Rat Osteoblasts. European Journal of Morphology
39:10-18.
Hurley MM, Marie PJ., Florkiewicz R. (2002) Fibroblast Growth
Factor and Fibroblast Growth Factor Receptor Families in Bone.
In Principles of Bone Biology, ed. by J. Bilezikian, L.G. Raisz
and G. Rodan. Academic Press pp 825-851.
Zhang X, Sobue T, Hurley MM. (2002) FGF-2 Increases Colony
Formation, PTH receptor, and IGF-1 mRNA in mouse Marrow Stromal
Cells. Biochem, Biophys. Res. Comm, 290:526-531.
Sobue T, Zhang X, Florkiewicz RZ, Hurley MM. (2001)
Interleukin-1 Regulates FGF2 mRNA and localization of FGF-2
protein in human osteoblasts. Biochem, Biophys. Res. Comm,
286:33-40. |
