Areas of Interest
The primary interests of my group are the molecular signals
which control the expression and function of mRNA molecules. We
study RNA polymerase II promoters and the mechanisms of
splicing, polyadenylation and RNA export from the nucleus to the
cytoplasm. A recent area of interest has been the mechanism of
action of naturally-occurring antisense RNA regulation in
mammalian cells. Our preferred model system is the mouse polyoma
virus, but we are also using other systems, including histone
genes, the HIV-1 virus and yeast.
Lab Rotation Projects
We study the consequences of double strand RNA in cells,
especially with regard to what happens in the nucleus. Our work
includes studies on RNA editing, the nucleocytoplasmic transport
of RNA molecules, and mechanisms by which dsRNAs can lead to
gene silencing. Rotation projects would most likely involve
molecular studies on proteins we have identified that play
important roles in dsRNA metabolism.
Selected Publications
Q. Wang and G.G. Carmichael: RNA Silencing. The Biologist, in
press.
Q. Wang and G.G. Carmichael: Effects of length and location
on the fate of dsRNA. Microbiol. Molec. Biol. Rev., in press.
Z. Zhang and G.G. Carmichael: Methods for the analysis of
adenosine-to-inosine in RNA (2004) Methods in Molec. Biol., 257:
75-83.
G.G. Carmichael: Antisense starts making more sense (2003)
Nature Biotechnology 21: 371-372.
Z. Zhang and G.G. Carmichael: Mechanism of action of
antisense RNA in eukaryotic cells. (2003) In, “Cancer Drug
Discovery and development: Nucleic Acid Therapeutics in Cancer”,
(A. Gewirtz, ed.), Humana Press, Totowa, NJ, pp. 89-104.
G.G. Carmichael: Silencing viruses with RNA. (2002) Nature
418: 379-380.
Y. Huang and G.G. Carmichael: "Nucleocytoplasmic mRNA
transport". (2001) In, Results and Problems in Cell
Differentiation, (D. Richter, ed.) vol. 34, pp. 140-155,
Springer-Verlag, Berlin.
L.E. Maquat and G.G. Carmichael: Quality control of mRNA
function. (2001), Cell 104: 173-176.
Z. Zhang and G.G. Carmichael: The fate of double-stranded RNA
in the nucleus: A multiprotein complex containing the inosine-specific
RNA-binding protein p54nrb mediates the nuclear retention of
A-to-I edited RNAs. (2001) Cell 106: 465-475. |
