Areas of Interest
Second Harmonic Generation (SHG) Imaging studies of fibrous
structural proteins including collagen, keratin, and myosin.
Aspects include studying macromolecular, long -range order on
the micron scale as well as quantifying the non-linear optical
properties. The emphasis is on using SHG as a diagnostic for
collagen and amyloid related diseases as well as possible
photonic device construction from these materials.
Micro and nanofabrication of biologically relevant materials
using multi-photon excitation. Applications are in the areas of
tissue engineering, microfluidics, sustained release and
biosensing. Development and optimization of new photochemistries,
and physical and optical characterization of fabricated
structures. Implementation of new non-linear optical schemes to
produce minimum feature sizes in the 50-100 nm range.
Photophysics of gold and silver nanoparticles linked to
environmentally sensitive dyes. The origin of the non-linear
optical properties are investigated by high-resolution SHG and
multi-photon excited fluorescence imaging by varying colloid
size, excitation wavelength, chromophore, and by polarization
anisotropy analysis. A goal of this work is the application of
these particles as chemical sensors in biological systems.
Development of novel nonlinear microscopes and methods for
imaging cells and cellular membranes including two and three
photon excited fluorescence, multi-photon fluorescence lifetime
imaging, and multi-photon photoactivation/photobleaching, second
harmonic generation and third harmonic generation in both
wide-field and near-field configurations.
Selected Publications
S. Basu and P.J. Campagnola, “Enzymatic Activity of Alkaline
Phosphatase inside Protein and Polymer Structures Fabricated via
Multi-photon Excitation” Biomacromolecules, in press
S. Basu, C.W. Wolgemuth, and P.J. Campagnola, “Measurement of
Normal and Anomalous Diffusion of Dyes within Protein Structures
Fabricated via Multi-photon Excited Crosslinking,” J. Phys. Chem
B, submitted.
A.C. Millard, P.J. Campagnola, W.A. Mohler, A. Lewis, and
L.M. Loew, “Second Harmonic Imaging Microscopy” Methods in
Enzymology, 361, 47-69 (2003).
P.J. Campagnola, A.C. Millard, and W.A. Mohler, “Second
Harmonic Generation Imaging Microscopy of Endogenous Structural
Proteins” Methods, 29, 97 – 109 (2003).
William A. Mohler and P.J. Campagnola, “Nonlinear optical
spectroscopy and imaging of structural proteins in living
tissues” Optics and Photonics News, 14, 40-45 (2003).
R.M. Brown, Jr, A.C. Millard, and P.J. Campagnola,
“Macromolecular Structure of Cellulose Studied by Second
Harmonic Generation Imaging Microscopy” Optic Lett,22, 2207-2209
(2003).
L.M. Loew and P.J. Campagnola, “Exact change required: Second
harmonic imaging microscopy visualizes distinct biomolecular
arrays in live cells tissues and organisms” Nature Biotech, 11,
1356-1360, (2003).
L.M. Loew, P.J. Campagnola, A. Lewis, and J.P. Wuskell,
“Confocal and Non-linear Optical Imaging of Potentiometric Dyes”
Methods in Cell Biology, 70, 429-453 (2002).
Paul J. Campagnola, Mark Terasaki, P.E. Hoppe, C.J. Malone,
and W. A. Mohler, "3-Dimesional High-Resolution Second Harmonic
Generation Imaging of Endogenous Structural Proteins in
Biological Tissues" Biophys J., 81, 493-508(2002).
J. D. Pitts, A. R. Howell, I. Banerjee, J. Wang, S. L.
Goodman, and P. J. Campagnola, ” New photoactivators for
multi-photon excited 3 dimensional sub-micron crosslinking of
proteins.” Photochem Photobiol, 76 135-144 (2002).
P. J. Campagnola, Heather A. Clark, William A. Mohler, Aaron
Lewis, and Leslie M. Loew, "Second Harmonic Imaging Microscopy
of Living Cells" J. Biomedical Optics, 6, 277-286 (2001). |
